Research Grade Testing

As part of our research program we routinely test clinical samples for particular molecules that can also be informative to clinical care. For sample testing or for more information, please contact us.

Adenosine Deaminase 2 Activity

Molecular structure of Adenosine Deaminase 2

Adenosine deaminase 2 is an extracellular growth factor and enzyme that breaks down the molecules adenosine and 2’-deoxyadenosine. A “deficiency in ADA2 (DADA2)” causes abnormal function of organs, tissues and particularly blood vessels which can be life threatening.

We measure ADA2 enzymatic activity (Diazyme Laboratories) and concentration in plasma or sera and compare to in-house established normative ranges for healthy children. Reference: Bowers et. al. Pediatr. Rheumatol. Online. J. (2020) 18(1): 54.

Type I Interferon (IFN) Score

Molecular structure of Protein IFNα2

Type I interferons (IFN) have important roles in many immune-mediated inflammatory diseases (IMIDs) and are a relatively new therapeutic target.

To infer Type I IFN activity, we measure the relative expression of six interferon-stimulated genes (ISGs) in whole blood (collected in Tempus or PAXGene RNA tubes) and calculate an interferon score as per methods by Rice et. al. ​J. Clin. Immunol. (2017) 37: 123-32.

Anti-Neutrophil Cytoplasmic Antibodies (ANCA) Titers

Schematic of Direct ELISA of antibodies against PR3

ANCA are autoantibodies, mostly IgG, often present in individuals with autoimmune vasculitis. The antibodies are directed towards neutrophil proteins proteinase 3 and/or myeloperoxidase.

We perform enzyme linked immunoassays (ELISA) on human sera to establish titers (U/mL) of anti-PR3 (Orgentec), anti-MPO (BioRad Laboratories) antibodies and anti-LAMP2 (in-house assay) antibodies.